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KMID : 0362220130400040359
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Journal of The Korean Research Society for Dental Materials
2013 Volume.40 No. 4 p.359 ~ p.365
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The effect of interconnective micropores of biphasic calcium phosphate scaffold on osteoblast cellular behavior
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Lee Mun-Hwan
You Chang-Kook
Lee Hyo-Jin
Kwon Tae-Yub
Kim Kho-Han
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Abstract
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The objective of this study was to evaluate the effect of interconnective micropore on osteoblast proliferation and spreading. The micropores of scaffold were observed by field emission scanning electron microscope. Surface roughness of slurry coated surface was determined by the atomic force microscope. Contact angle goniometer was used to evaluate differences in contact angle between surfaces. MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay was used to measure the cell proliferation. The surface roughness of microporous specimens was higher than that of non-microporous specimen. The contact angles of microporous specimens were lower than that of non-microporous specimen. Cell spread was greater on microporous scaffolds than non-microporous scaffolds. In addition, cell proliferation was increased when cells were cultured on microporous scaffold. The result demonstrated that interconnective micropores of biphasic calcium phosphate scaffold generally improved the response of osteoblast in-vitro. This behavior could be explained by surface roughness and hydrophilicity.
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KEYWORD
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Interconnective micropores, Biphasic calcium phosphate scaffold, Roughness, Contact angle, Proliferation, Spreading
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